Figure 6.

DHA effectively reduces transactivating capacity of LXRα. Hepatocytes were transfected with plasmids expressing Gal4-LXRα, Gal4 promoter luciferase construct, and null renilla control. Following transfection, cells were incubated with T0901317 (0,1, 5, or 10 μM) in the presence or absence of BSA (0.15%) or DHA (100 μM). After 24 hours of treatment, cells were lysed, luciferase activity measured and normalized to renilla luminescence. Data are expressed as the fold change in normalized luciferase from BSA + vehicle control and are the mean of at least five hepatocyte preparations (n ≥ 5). * P ≤ 0.05 vs. T0901317 + BSA