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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Dev Biol. 2009 Sep 22;336(1):30–41. doi: 10.1016/j.ydbio.2009.09.022

Figure 3. Co-expression of G8, MyoD mRNA and Noggin in the developing eye.

Figure 3

The eyes of stage 18 (A, C, E, G, H, M and O) and stage 24 (B, D, F, I, J, K, L, N and P) embryos were sectioned and stained with H & E (A and B) or double labeled with the G8 MAb (G8 A) (green) and dendrimers to MyoD mRNA (MyoD m) (red) or Noggin mRNA (Nog m) (red), or an antibody to Noggin protein (Nog P) (green) and dendrimers to MyoD mRNA. Nuclei were counterstained with Hoechst dye (blue). The overlap of red and green appears yellow-white in merged images. Cells co-expresssing G8 and MyoD mRNA were present in the lens (LNS) (E), anterior optic cup (AOC) (F), the retinal layer of the posterior optic cup (POC) (G), and periocular mesenchyme (POM) (H). Cells expressing the G8 antigen also expressed Noggin mRNA in the lens (I), anterior and posterior optic cup (J and K), and periocular mesenchyme (L). A few cells with Noggin mRNA were not stained with G8 in the lens (arrowhead in J). Noggin protein and MyoD mRNA were co-localized in some cells of the lens (M), anterior optic cup (arrow in N), posterior optic cup (O), and periocular mesenchyme (P). Other cells labeled for Noggin protein did not contain detectable levels of MyoD mRNA (MyoD m-) in the lens and optic cup (arrows in C and D). Scale bar: 56 µm in A and B; 13 µm in C–P.