Table 5.
Retina | Lens | POM | ||||
---|---|---|---|---|---|---|
Embryo | left | right | left | right | left | right |
#7 | 3 Nog+/MyoD+ | 0 Nog+ | 0 Nog+ | 0 Nog+ | 0 Nog+ | |
3 Nog+/MyoD− | 1 Nog+/MyoD− | |||||
#9 | 14 Nog+/G8+ | 4 Nog+/G8+ | 2 Nog+/G8+ | 1 Nog+/G8+ | 0 Nog+ | 0 Nog+ |
1 Nog+/G8− |
All sections through the stages 23–24 eyes of embryos ablated at stage 2 were double labeled for the G8 antigen or MyoD mRNA and Noggin mRNA or Noggin protein. The number of cells expressing Noggin (Nog) with or without MyoD or G8 was determined in the retina, lens and periocular mesenchyme (POM). Ablation of MyoD+ cells in the epiblast reduced or eliminated Noggin+ cells in ocular tissues. Fewer Noggin+ cells were present in the right eyes of ablated embryos than the left eyes.