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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Cancer Res. 2009 Oct 13;69(21):8386–8394. doi: 10.1158/0008-5472.CAN-09-1504

Figure 1.

Figure 1

Paclitaxel negatively regulates survival and expression of AR targeted genes in 22Rv1 CRPC cells. A, Effect of paclitaxel on apoptosis. 22Rv1 cells were treated with different concentrations of paclitaxel. After 48 h of treatment, cells were harvested, fixed, stained with propidium iodide (PI) and FACS analysis was performed and sub-G1 cells were considered dead. B, C, Effect of paclitaxel on expression of AR target genes. 22Rv1 cells were treated with different concentrations of paclitaxel. After 48 h of treatment, cells were harvested for mRNA and protein analysis. The expression levels of PSA, Nkx3.1 and maspin mRNA were examined by semiquantitative RT-PCR analysis (B, upper panel). GAPDH mRNA was used as an internal control (B, upper panel). The level of the AR protein was assessed by Western blot (B, lower panel). The relative mean mRNA expression level of AR regulated genes was measured by quantitative real-time RT-PCR in triplicate. Expression levels are relative to vehicle treatment, which was arbitrarily set to 1 (C).