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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: J Immunol. 2009 Nov 4;183(11):7031–7038. doi: 10.4049/jimmunol.0802665

Figure 5.

Figure 5

Treatment of estrogen-responsive WT276 cells with E2 or overexpression of ERα stimulated the activity of 202-luc-reporter. (a) Sub-confluent cultures of WT276 cells in a 6-well plate were transfected with 202-luc-reporter plasmid (2.5 μg) along with pRL-TK (0.5 μg) plasmid using calcium phosphate precipitation method. 24 h after transfections, cells were either treated with ethanol (vehicle) or E2 (5 or 10 nM). 40–45 h after transfections, cells were processed for dual luciferase activity. (b) Sub-confluent cultures of WT276 cells in a 6-well plate were transfected with 202-luc-reporter plasmid (2.5 μg), pRL-TK (0.5 μg) plasmid along with either empty vector or a plasmid encoding ERα using calcium phosphate precipitation method. 24 h after transfections, cells were either treated with ethanol (vehicle) or E2 (5 or 10 nM). 40–45 h after transfections, cells were processed for dual luciferase activity. (c) Sub-confluent cultures of NIH 3T3 cells in a 60 mm plate were transfected either empty vector or a plasmid encoding ERα using calcium phosphate precipitation method. 40–45 h after transfections, cells were processed for immunoblotting using antibodies specific to the indicated proteins.