Brain microvessels (a-b) or brain homogenate (c-d) were obtained from mice trerated with subacute LPS. (a) Western blot analysis for RAGE showed no effect of LPS on the protein levels in brain microvessels of this blood-to-brain transporter of Aß. (b) Western blot analysis for 515 kDa subunit of the BBB Aβ clearance transporter, LRP-1, in isolated mouse brain microvessels from mice treated with saline, LPS and indomethacin as indicated (n = 20-22 cortices/microvessel isolation with 1 isolation/band, analyzed 5 times). LPS decreased LRP protein expression (*p<0.05) but indomethacin was without effect. (c) LRP mRNA and LRP protein (d) levels from whole brain in mice treated with saline (n = 6) or LPS (n = 6-7). LPS increased both mRNA (*p<0.05) and protein (**p<0.01) expression in whole brain, indicating upregulation of neuronal LRP.