. Author manuscript; available in PMC: 2010 Nov 24.

Published in final edited form as: Biochemistry. 2009 Nov 24;48(46):11032–11044. doi: 10.1021/bi901597j

Table 3.

Activity profiles of CpaD with N-terminal Trp-containing dipeptides.

Substrate	Product Identification		observed v_i (min⁻¹)^a
Substrate	[M+H]⁺cal.	[M+H]⁺obs.	observed v_i (min⁻¹)^a
9 (H-Trp-Ala-OH)	344.1974	344.1977	0.34 ± 0.06
10 (H-Trp-Gly-OH)	330.1818	330.1812	0.16 ± 0.05
11 (H-Trp-Val-OH)	304.1661	304.1665	0.31 ± 0.07
12 (H-Trp-Leu-OH)	386.2444	386.2449	0.18 ± 0.04
13 (H-Trp-Phe-OH)	352.1661	352.1666	0.11 ± 0.03
14 (H-Trp-Tyr-OH)	368.1610	368.1613	0.22 ± 0.05

Saturation kinetics could not be achieved even with 1 mM of 9–14 as substrates, therefore observed maximum initial velocity for these substrates is shown.