Table 4.
Relative activity profiles of polar/charged CpaD mutants targeted by examination of conserved motifs in the Trp-DMAT family.a
| Entry | CpaD protein | virel |
|---|---|---|
| 1 | E93L | (6.8 ± 0.7) × 10−4 |
| 2 | S95A | 0.92 ± 0.11 |
| 3 | N97L | 0.63 ± 0.15 |
| 4 | R104E | (2.1 ± 0.3) × 10−2 |
| 5 | D119L | –– |
| 6 | D181L | 0.47 ± 0.03 |
| 7 | K191E | (2.7 ± 0.5) × 10−4 |
| 8 | T203V | 0.96 ± 0.08 |
| 9 | D250L | (5.1 ± 0.6) × 10−2 |
| 10 | R258E | 0.92 ± 0.12 |
| 11 | K260E | (3.2 ± 0.4) × 10−4 |
a
virel is the relative observed maximum initial velocity of each mutant CpaD to that of wild type, which is defined as 1. Mutants in bold are those with a notable decrease in relative activity. D119L could not be overproduced in soluble form for characterization.