Fig. 2. Presence of the anti-sense transcript through the time course of growth in a wild-type strain.
A. RT-PCR results for AS- and S-fliR transcripts monitored through 8 hrs of growth in LB in the wild-type strain. 200 ng of RNA were used for PCR reactions, with 29 cycles of amplification.
B. As in A except in an isogenic ΔrcsB mutant QW434.