TABLE 1.
Strains and plasmids used
| Strain/plasmid | Genotypea | Source (Ref.) |
|---|---|---|
| Strains | ||
| 14028 | Wild type S. enterica | |
| QW119 | igaA* | Wang et al 2007 |
| QW422 | ΔfliR::kan, igaA* | This study |
| QW434 | ΔrcsB | Wang et al 2007 |
| QW441 | ΔrcsA, igaA* | Wang et al 2007 |
| QW442 | ΔrcsB, igaA* | Wang et al 2007 |
| QW471 | ΔfliQR, igaA* | This study |
| QW555 | ΔRA(49–88) | This study |
| QW556 | ΔRA(1–48), igaA* | This study |
| QW593 | anti-fliFLAG-R, igaA* | This study |
| QW594 | anti-fliR-FLAG, igaA* | This study |
| QW597 | pBAD33 | This study |
| QW610 | pfliPQRBAD | This study |
| QW605a | pLfliQRBAD | This study |
| QW663 | ΔtopA::kan | This study |
| QW667 | ΔPrcsA | This study |
| QW668 | ΔtopA | This study |
| QW669 | AS-fliR::T (TrrnB at bp 10 after fliR TAA) | This study |
| QW671 | PAS-fliR# (atatat @ -10 mutated to cgcgcg) | This study |
| QW672 | ΔPrcsA, igaA* | This study |
| QW673 | AS-fliR::T, igaA* | This study |
| QW674 | PAS-fliR#, igaA* | This study |
| QW675 | ΔtopA::kan, ΔPrcsA | This study |
| QW676 | ΔtopA::kan, ΔrcsB | This study |
| QW695 | ΔrpoS | This study |
| QW698 | SV4989 (rcsC P484L) | Garcia-Calderón et al 2005 |
| QW699 | AS-fliRSTOP2(186th n.t. C changed to A) | This study |
| QW700 | AS-fliRSTOP2, igaA* | This study |
| QW701 | AS-fliRSTOP1(13th n.t. A changed to T) | This study |
| QW702 | AS-fliRSTOP1, igaA* | This study |
| Plasmids | ||
| pBAD33 | vector | Guzman et al 1995 |
| pfliPQRBAD | fliPQR in pBAD33 | This study |
| pLfliQRBAD | fliQR in pBAD33 | This study |
refers to the point mutation described in the indicated study. Δ followed by :: refers to gene deletion concomitant with substitution of indicated antibiotic resistance cassette. Primers used for various manipulations are listed in Supp. Table 1, and extent of deletions created in Suppl. Table 2. RA refers to the intergenic region between fliR and rcsA, and deletion numbers refer to base pairs to the 3′ of fliR after the stop codon TAA. FLAG subscripts refer to epitope tags placed either at the 5′ or 3′ end of the indicated genes. L and BAD superscripts refer to fliL or PBAD promoters driving sense or anti-sense transcription of the indicated genes, respectively. T refers to the 426 bp rrnB T1T2, # to the 6 bp substitution at −10 of AS promoter. The stop codon numbering refers to the putative anti-FliR ORF that starts in Val; G of GTA is 17th nt after TAA of fliR. See Experimental Procedures for details.