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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Cell Motil Cytoskeleton. 2009 Dec;66(12):1048–1056. doi: 10.1002/cm.20407

Figure 1. Pfn1-induced clustering of MDA-231 cells is reversible.

Figure 1

A) Relative levels of endogenous vs exogenous Pfn1 in GFP-Pfn1 expressing cells. B) Pfn1 immunoblot showing strong suppression of endogenous and exogenous Pfn1 in GFP-Pfn1 expressers 72 hours after Pfn1-siRNA treatment (GAPDH blot serves as the loading control). C) Phase-contrast micrographs of GFP-Pfn1 expressers 72 hours after treatment with either control or Pfn1-siRNA (morphology of untransfected GFP and GFP-Pfn1 expressers are shown side-by-side for comparison). D) Vimentin immunoblot of GFP and GFP-Pfn1 expressers (GAPDH blot serves as the loading control). Scale — 100 μm.