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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Cell Motil Cytoskeleton. 2009 Dec;66(12):1048–1056. doi: 10.1002/cm.20407

Figure 3. Perturbing Pfn1 expression alters actin cytoskeletal structures in MDA-231 and normal HMEC.

Figure 3

A) Phalloidin staining shows circumferential F-actin cables in GFP-Pfn1 expressers (right panel — see arrows), but not in GFP expressers (left panel). B) β-catenin immunostaining (left and middle panels) of GFP-Pfn1 expressers of MDA-231 cells 20 min after either DMSO (vehicle control) or 500 nM CD treatment (arrows show junctional localization). The right most panel shows phalloidin staining of CD-treated GFP-Pfn1 expressers. C) Phalloidin staining of HMEC treated with control and Pfn1-siRNA (Pfn1 immunoblot next to the micrographs shows siRNA—mediated downregulation of Pfn1 expression; GAPDH blot serves as the loading control). D) Phalloidin (left panel) and β-catenin (right panel) staining of GFP-Pfn1-H119E expressers of MDA-231 cells (Scale bar — 20 μm).