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. Author manuscript; available in PMC: 2010 Oct 1.

Published in final edited form as: Pancreas. 2009 Oct;38(7):782–790. doi: 10.1097/MPA.0b013e3181a85999

Combination Gemcitabine and Proton Radiation in PANC-1 and MIA PaCa-2 cell lines. (A) PANC-1 and (B) MIA PaCa-2 cells were treated using 10 Gy of proton radiation and 10 μM gemcitabine after which they were harvested and analyzed for DNA content by propidium iodide staining and flow cytometry at 24 hr, 48 hr, and 72 hr. Percentages of apoptotic cells with hypodiploid (sub-G1) DNA content as well as those in G₀/G₁, S, G₂/M and polyploid are indicated per each condition tested. Data are the mean ± SD of three independent experiments (*p<0.01). Detergent-solubilized extracts of (C) PANC-1 and (D) MIA PaCa-2 cells treated with 10 Gy of proton radiation and 10 μM gemcitabine were analyzed at 48h for reactivity with antibodies for survivin and μ-actin or GAPDH (loading control), by Western blotting. (E) PANC-1 and (F) MIA PaCa-2 membranes were stripped and reprobed with antibodies for XIAP after which densitometric fold changes for each were indicated below. Molecular-weight (M_r) markers in kilodaltons are shown on the left.

Combination Gemcitabine and Proton Radiation in PANC-1 and MIA PaCa-2 cell lines. (A) PANC-1 and (B) MIA PaCa-2 cells were treated using 10 Gy of proton radiation and 10 μM gemcitabine after which they were harvested and analyzed for DNA content by propidium iodide staining and flow cytometry at 24 hr, 48 hr, and 72 hr. Percentages of apoptotic cells with hypodiploid (sub-G1) DNA content as well as those in G₀/G₁, S, G₂/M and polyploid are indicated per each condition tested. Data are the mean ± SD of three independent experiments (*p<0.01). Detergent-solubilized extracts of (C) PANC-1 and (D) MIA PaCa-2 cells treated with 10 Gy of proton radiation and 10 μM gemcitabine were analyzed at 48h for reactivity with antibodies for survivin and μ-actin or GAPDH (loading control), by Western blotting. (E) PANC-1 and (F) MIA PaCa-2 membranes were stripped and reprobed with antibodies for XIAP after which densitometric fold changes for each were indicated below. Molecular-weight (M_r) markers in kilodaltons are shown on the left.