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. 2009 Nov 9;3:19. doi: 10.3389/neuro.04.019.2009

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Copyright © 2009 Nathanson, Jappelli, Scheeff, Manning, Obata, Brenner and Callaway.

This is an open-access article subject to an exclusive license agreement between the authors and the Frontiers Research Foundation, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are credited.

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Percentage of virus label in inhibitory neuron subtypes. Viruses expressing GFP were injected into mouse somatosensory cortex and stained for inhibitory neuron markers calretinin (CR), parvalbumin (PV), somatostatin (SST), vasoactive intestinal polypeptide (VIP) and neuropeptide Y (NPY). Percentages were calculated by dividing the number of co-labeled cells (GFP and cell-type specific antibody) by the total number of antibody labeled cells in the injected region. The percentages reflect the combined labeling in cortical layers 2/3–6 and the standard deviations show the labeling distributions treating each section as an independent observation. Sections were counted from a single animal for AAV-fPV, and from three animals for all others. NPY label was not counted for AAV-fPV and AAV-fCR. At least 170 cells were counted for each vector/antibody combination (see Materials and Methods).