Table 1.
AAV and LV vectors with fugu promoters.
| Gene/promoter | Symbol(a) | Transgene | Genomic titer (genomes/ml) | Promoter length(b) | Target cell-type | Glia expression(c) | Excitatory neuron expression(c) | Inhibitory neuron expression(c) |
|---|---|---|---|---|---|---|---|---|
| Calretinin | AAV-fCR | GFP | 9.70E + 10 | 2595 | Inhibitory neuron subtype (CR) | + | − | + |
| Neuropeptide Y | AAV-fNPY | GFP | 6.70E + 10 | 2605 | Inhibitory neuron subtype (NPY) | − | − | +++ |
| Somatostatin | AAV-fSST | GFP | 1.40E + 11 | 2597 | Inhibitory neuron subtype (SST) | − | − | +++ |
| RFP | 5.92E + 11 | − | + | +++ | ||||
| Parvalbumin | AAV-fPV | GFP | 1.20E + 11 | 2395 | Inhibitory neuron subtype (PV) | − | ++ | ++ |
| Secreted Frizzled Receptor Protein 2 | AAV-fSFRP2 | GFP | 2.70E + 11 | 1218 | Inhibitory neuron subtype (SST) | + | − | + |
| Titin Cap | AAV-fTCAP | GFP | 2.00E + 11 | 2656 | Inhibitory neuron subtype (PV) | + | + | + |
| α-calcium/calmodulin-dependent protein kinase II | AAV-fCAMK | GFP | 4.5E + 10 | 2567 | Excitatory neurons | − | ++ | ++ |
| Calretinin | LV-fCR | GFP | 2.01E + 10 | 6326 | Inhibitory neuron subtype (CR) | − | + | + |
| Neuropeptide Y | LV-fNPY | GFP | 4.51E + 10 | 6443 | Inhibitory neuron subtype (NPY) | − | + | + |
| Somatostatin | LV-fSST | GFP | 4406 | Inhibitory neuron subtype (SST) | − | + | + | |
| Parvalbumin | LV-fPV | GFP | 3.54E + 10 | 2893 | Inhibitory neuron subtype (PV) | − | ++ | + |
(a) Adeno-associated virus (AAV) and lentivirus (LV). Fugu derived sequences are denoted by the prefix “f”.
(b) Length of the promoter used in the virus (bases).
(c) Scoring is from mouse injections to somatosensory cortex.