Table 1.

Unfolding Transition Temperatures of NKX3.1 Constructs^a

NKX3.1 construct	temp (°C)	protein/peptide mixtureb	temp (°C)
114–184	40	114–184/AD	34
97–184	40	114–184/phospho-AD	36
75–184	42	114–184/ADSI	42
75–184-T89E	43
75–184-T93E	43
75–184-T89E/T93E	44
1–184	41

^aAll temperature values accurate to within approximately ±1 °C. The buffer for all samples consisted of 20 mM MES and 50 mM NaCl (pH 6.5).

^bIn 100 μM NKX3.1 (114–184), 1.6 mM AD, phospho-AD, or ADSI peptide.