Skip to main content
. Author manuscript; available in PMC: 2010 Nov 15.
Published in final edited form as: Cancer Res. 2009 Oct 20;69(22):8700–8709. doi: 10.1158/0008-5472.CAN-09-1145

Figure 3. IL-12 signaling blocks Treg expansion indirectly.

Figure 3

A. Representative flow cytometric plots show the percentages of CD4+Foxp3+ and CD4+Foxp3− T cells in the spleens of naïve WT C57BL/6J (B6) mice and strain-matched IL-12 receptor β2-deficient (IL12Rβ2-/-) mice. 10 mice in each genotype were examined in 3 independent experiments, with similar results. B. RMAS tumor cells were cultured with WT B6 splenocytes or IL12Rβ2-/- splenocytes for 5 days. Summary data from 2 independent experiments are shown. C and D. Purified WT and IL12Rβ2-/- Treg cells were labeled with CFSE and mixed into co-cultures of tumor cells with either WT splenocytes (C) or IL12Rβ2-/- splenocytes (D) for 5 days, and expansion of the CFSE-labeled Treg cells are summarized for 2 independent experiments. Treg expansion is presented as fold-change of the final yield over initial input as described in Figure 1. Data points are shown as mean ± SD; Two-tailed t-tests were used to determine statistical significance (*P<0.05, **P<0.01, ***P<0.001).