Abstract
Jensen, Roy A. (The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston) and Felix L. Haas. Electrokinetics and cell physiology. II. Relationship of surface charge to onset of bacterial competence for genetic transformation. J. Bacteriol. 86:79–86. 1963.—A reliable cell fractionation scheme, which is sensitive to the electrokinetic properties of Bacillus subtilis cells, has been described in detail. Recipient cell populations, characterized by a wide range of competency for transformation to independence of nutritional markers, were subjected to electrokinetic fractionation. Results indicated that (i) physiological competency is directly related to the electrical charge on the cell surface, (ii) newly competent cells carry a maximal negative charge, (iii) the newly competent cell appears with spontaneous abruptness, (iv) a kinetic flow of competent cells from the highly charged fractions to the lower charged fractions indicates the progressive loss of the surface charge maintained by a competent cell, and (v), by token of the latter statement, cells competent to undergo transformation do so within a range of surface-charge values.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CLARK A. J., ADELBERG E. A. Bacterial conjugation. Annu Rev Microbiol. 1962;16:289–319. doi: 10.1146/annurev.mi.16.100162.001445. [DOI] [PubMed] [Google Scholar]
- COMOLLI R., MACCACARO G. A. Surface properties correlated with sex compatibility in Escherichia coli. J Gen Microbiol. 1956 Aug;15(1):121–132. doi: 10.1099/00221287-15-1-121. [DOI] [PubMed] [Google Scholar]
- JENSEN R. A., HAAS F. L. ELECTROKINETICS AND CELL PHYSIOLOGY. 1. EXPERIMENTAL BASIS FOR ELECTROKINETIC CELL STUDIES. J Bacteriol. 1963 Jul;86:73–78. doi: 10.1128/jb.86.1.73-78.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JENSEN R. A., HAAS F. L. Quantitative studies on the expression of a transformed character in Bacillus subtilis. Biochim Biophys Acta. 1962 Dec 31;61:963–969. doi: 10.1016/0926-6550(62)90012-9. [DOI] [PubMed] [Google Scholar]
- MACCACARO G. A. Cell surface and fertility in Escherichia coli. Nature. 1955 Jul 16;176(4472):125–126. doi: 10.1038/176125a0. [DOI] [PubMed] [Google Scholar]
- SMITH S. M., STOCKER B. A. Colicinogeny and recombination. Br Med Bull. 1962 Jan;18:46–51. doi: 10.1093/oxfordjournals.bmb.a069934. [DOI] [PubMed] [Google Scholar]