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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Protein Expr Purif. 2009 Jun 28;69(1):83–90. doi: 10.1016/j.pep.2009.06.016

Table I.

Yeast strains and plasmids used in this work.

Strain/Plasmid Description Source
Yeast strains
YBC-1 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 Langelier et al., 2005
YBC-9 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 Langelier et al., 2005
YBC-10 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 pFL39-RPB2 This study
YBC-15 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 pFL39-rpb2-R504A This study
YBC-16 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 pFL39-rpb2-D505A This study
YBC-17 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 pFL39-rpb2-R512A This study
YBC-18 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 pFL39-rpb2-Q513A This study
YBC-19 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 pFL39-rpb2-R983Q This study
YBC-20 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 pFL39-rpb2-E1028Q This study
YBC-21 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 pFL39-rpb2-K510A This study
YBC-23 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 pFL39-rpb2-R1020Q This study
YBC-24 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 pFL39 This study
YBC-25 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pFL39-RPB2 This study
YBC-26 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pFL39-rpb2-R504A This study
YBC-27 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pFL39-rpb2-D505A This study
YBC-28 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pFL39-rpb2-K510A This study
YBC-29 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pFL39-rpb2-Q513A This study
YBC-30 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pFL39-rpb2-R983Q This study
YBC-31 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pFL39-rpb2-E1028Q This study
YBC-32 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 pFL39-RPB2 This study
YBC-33 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 pFL39-rpb2-R504A This study
YBC-34 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 pFL39-rpb2-D505A This study
YBC-35 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 pFL39-rpb2-K510A This study
YBC-36 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 pFL39-rpb2-R512A This study
YBC-37 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 pFL39-rpb2-Q513A This study
YBC-39 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 pFL39-rpb2-R983Q This study
YBC-40 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 pFL39-rpb2-R1020Q This study
YBC-41 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 pFL39-rpb2-E1028Q This study
YBC-42 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 pFL39 This study
YBC-44 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 pFL39-rpb2-R512C This study
YBC-45 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 pFL39-rpb2-E529A This study
YBC-46 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 pFL39-rpb2-K987R This study
YBC-47 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 pFL39-rpb2-K987Q This study
YBC-48 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 pFL39-rpb2-E529D This study
YBC-49 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 pFL39-rpb2-E529Q This study
YBC-50 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 pFL39-rpb2-R1020K This study
YBC-51 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 pFL39-rpb2-S1019F This study
YBC-52 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 pFL39-rpb2-K979R This study
YBC-53 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 pFL39-rpb2-K979Q This study
YBC-54 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 pFL39-rpb2-P528DG530A This study
YBC-55 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pYC2/CT-RPB2 pFL39-rpb2-G985AG988A This study
YBC-56 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pFL39-rpb2-E529A This study
YBC-57 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pFL39-rpb2-E529D This study
YBC-58 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pFL39-rpb2-S1019F This study
YBC-59 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pFL39-rpb2-P528DG530A This study
YBC-60 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pFL39-rpb2-E529Q This study
YBC-61 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pFL39-rpb2-G985AG988A This study
YBC-64 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 pFL39-rpb2-R512C This study
YBC-65 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 pFL39-rpb2-E529A This study
YBC-66 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 pFL39-rpb2-E529D This study
YBC-67 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 pFL39-rpb2-E529Q This study
YBC-68 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 pFL39-rpb2-P528DG530A This study
YBC-69 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 pFL39-rpb2-K979Q This study
YBC-70 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 pFL39-rpb2-K979R This study
YBC-71 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 pFL39-rpb2-K987Q This study
YBC-72 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 pFL39-rpb2-K987R This study
YBC-73 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 pFL39-rpb2-G985AG988A This study
YBC-74 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 pFL39-rpb2-S1019F This study
YBC-75 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 dst1::kanMX pYC2/CT-RPB2 pFL39-rpb2-R1020K This study
YBC-76 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pFL39-rpb2-R512A This study
YBC-77 MATa can1–100 his3–11,15 leu2–3,112 trp1–1 ura3–1 ade2–1 rpb2::LEU2 pFL39-rpb2-R512C This study
Plasmids
pYC2/CT URA3, ampR, CEN6, ARSH4 Sikorski and Hieter, 1989
pFL39 TRP1, ampR, CEN6, ARS Bonneaud etal., 1991