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. Author manuscript; available in PMC: 2010 Dec 31.
Published in final edited form as: J Immunol Methods. 2009 Oct 8;351(1-2):62–70. doi: 10.1016/j.jim.2009.09.011

Figure 4. Effect of different substrates on fibrocyte differentiation.

Figure 4

PBMC separated from heparin treated blood were cultured in FibroLife SFM for 5 days at 2.5 × 105 cells per ml on 8-well slides composed of “standard” soda lime glass (glass), positively charged glass (CC2), or plastic (Permanox) substrates. PBMC were similarly cultured in 96 well tissue culture plates (96 well). Results are mean ± SEM (n=3 separate donors).