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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Int J Cancer. 2010 Jan 1;126(1):81–89. doi: 10.1002/ijc.24696

Figure 2. KLF5 has no detectable effect on the function of estrogen in cell proliferation in ER-negative breast epithelial cells.

Figure 2

(A) ER-negative breast cancer cell line SK-BR-3 was subjected to 3H-TdR incorporation assay after transfection, following the same procedures as in Figure 1A. (B) Confirmation of KLF5 expression in SK-BR-3 cells after transfection, as detected by western blot analysis. (C) 3H-TdR incorporation in the MCF 10A human mammary epithelial cell line, which is immortal but non-tumorigenic, after knockdown of KLF5 by transfection of siRNA against KLF5 (SiK) or luciferase control (SiL) into cells. Total siRNA concentration was 200 nM, with 100 nM each for SiL and SiK in the group of SiL+SiK. (D) Confirmation of KLF5 knockdown in MCF 10A cells by western blot analysis. Asterisks indicate statistically significant differences in cell growth between cells transfected with negative control siRNA and cells transfected with KLF5 siRNA (P < 0.05).