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. Author manuscript; available in PMC: 2010 Dec 3.
Published in final edited form as: J Control Release. 2009 Aug 8;140(2):100–107. doi: 10.1016/j.jconrel.2009.08.001

Figure 3.

Figure 3

Fluorescence Intensity and number of molecules/microbubble based on FACSCalibur data of microbubbles conjugated with Alexa 647-MVCAM.A(429). (A) Fluorescence histograms of microbubbles conjugated with fluorescent antibody at the indicated concentrations (μg/107 microbubbles). Results from calibration beads were used to calculate the number of fluorescent molecules attached to the surface of microbubbles. (B) Table shows results of molecules/microbubble with respect to the fluorescent antibody concentration and microbubbles were incubated with. (C) MVCAM.A(429) site density per microbubble as a function of antibody concentration (μg/107 microbubbles). Background fluorescence values were subtracted from results; n=4, MFI –mean fluorescence intensity.