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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Gene Ther. 2009 Jul 30;16(11):1300–1313. doi: 10.1038/gt.2009.93

Figure 2. Activated B cells efficiently induce antigen-specific CD8+ and CD4+ T cell proliferation in vitro.

Figure 2

(A) OVA-specific CD8+ OT-1 or CD4+ OT-2 T cells were incubated for 72 hrs at indicated ratios with cognate peptide-pulsed irradiated freshly isolated B cells or B cells stimulated with indicated agents for 20 hrs. The extent of T cell proliferation was determined by thymidine incorporation assay. α-CD40, anti-CD40 antibodies; Ctrl, CpG/α-CD40 activated B cells not pulsed with peptide. Representative results of one of four independent experiments using peptide-pulsed or OVA-transgenic B cells are shown. (B), Proliferation of OT-1 cells following incubation with peptide-pulsed irradiated B cells stimulated with indicated agents for 20 hrs (B:T cell ratio 1:2). (C), Proliferation of OT-1 or OT-2 cells following incubation with peptide-pulsed DCs or CpG/CD40-activated B cells at APC:T cell ratio of 1:2 (lined bars) or 1:32 (solid bars). Error bars represent standard errors (SE).