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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Gene Ther. 2009 Jul 30;16(11):1300–1313. doi: 10.1038/gt.2009.93

Figure 6. Activated B cells do not efficiently home to secondary lymphoid organs.

Figure 6

(A) B cells were either unstimulated or stimulated with indicated agents for 20 hrs, labeled with CFSE, and 3×106 cells were injected I.V. into B6 mice. Four hours later, blood, spleen, peripheral (PLN) and mesenteric (MLN) lymph node cells, and Peyer’s patches (PP) were harvested and the percentage of CFSE+ CD19+ cells of total CD19+ cell population in each tissue was determined. Three mice per group; error bars represent SE. One of three similar experiments is presented. (B) Homing properties of unstimulated (left panel) or CpG/CD40-stimulated (right panel) B cells isolated from C57Bl/6 or selectin-knockout (Sel-l,p,e-/-) mice were analyzed as described in (A). (C) The structure of CD62L and CD62L-SR mutant. LD - lectin domain, EGF - epidermal growth factor-like domain, CR - short consensus repeats, TD - transmembrane domain, CD -cytoplasmic domain; arrow denotes cleavage site. (D) Activated B cells expressing sheddase-resistant mutant of CD62L (CD62L-SR) exhibit greater capacity to home to PLNs. CpG/CD40-activated B cells were co-transfected with plasmid expressing EGFP and either control empty plasmid, CD62L, or CD62L-SR-expressing plasmids and 5×105 transfected cells were injected intravenously. The frequency of CFSE+ B cells was determined in various organs 4 hrs post injection.