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. Author manuscript; available in PMC: 2010 Nov 13.
Published in final edited form as: Cell. 2009 Oct 29;139(4):693–706. doi: 10.1016/j.cell.2009.10.014

Figure 1.

Figure 1

Epigenetic switch from untransformed to a transformed phenotype

(A) Number of mammosphere formed/1000 seeded transformed (TAM-treated for 36h; mean ± SD) ER-Src cells during 12 serial passages.

(B) Levels of Src-Y419 phosphorylation (ELISA assay; mean ± SD) in untreated, and TAM-treated (36h), and 1st, 2nd, 4th, and 8th generation mammospheres derived from TAM-treated ER-Src cells.

(C) Kinetics of cellular transformation as a function of TAM exposure. ER-Src cells were treated with 1 μM TAM for the indicated times, and then analyzed for cellular transformation (defined by morphological changes) 24, 48 and 72h post TAM treatment.

(D) Phase-contrast images and Src-Y419 phosphorylation levels (ELISA assay; mean ± SD) in ER-Src cells 36 and 120h post TAM treatment. Cells were treated with TAM for 36h and then TAM was removed from the medium.