Skip to main content
. Author manuscript; available in PMC: 2010 Nov 13.
Published in final edited form as: Cell. 2009 Oct 29;139(4):693–706. doi: 10.1016/j.cell.2009.10.014

Figure 2.

Figure 2

Src induces an inflammatory response mediated by NF-κB

(A) Heatmap representation of RNA levels for the indicted inflammatory genes at the indicated time points after TAM induction.

(B) IL6 levels (ELISA assay; mean ± SD)) at the indicated times after TAM treatment. The black arrows show the biphasic induction of IL6.

(C) Phosphorylation status of IκBα-serine 32 (ELISA assay) in cells treated with TAM for the indicated times.

(D) NF-κB/p65 ActivELISA assay (NF-κB nuclear localization) cells treated with TAM for the indicated times.

(E) Soft agar colony assay (mean ± SD) in untreated and TAM-treated cells in the presence or absence of NF-κB inhibitors (5 μM BAY-117082 and 6 μM JSH-23).