. Author manuscript; available in PMC: 2010 Dec 1.

Published in final edited form as: Biochim Biophys Acta. 2009 Jul 25;1791(12):1125–1132. doi: 10.1016/j.bbalip.2009.07.004

Table 2.

Intracellular apo A-I and apo A-II are on distinct particles. ^a

	HDL		HepG2 cell lysate		Huh7 cell lysate
	free	bound	free	bound	free	bound
Apo A-I	0.34 + 0.05	0.66 + 0.05	0.98 + 0.03	0.02 + 0.03	0.94 + 0.09	0.06 + 0.09
Apo A-II	0.06 + 0.04	0.94 + 0.04	0.01 + 0.01	0.99 + 0.01	0.08 + 0.11	0.92 + 0.11
Apo E	0.22 + 0.16	0.78 + 0.16	0.40 + 0.10	0.60 + 0.10	0.41 + 0.07	0.59 + 0.07

Plasma and cells lysates were fractionated on TPS columns, as in Figure 4. Western blot band intensity was quantified on a Storm 840 imaging system with ImageQuant TL software, and the relative amount of each apolipoprotein in the free and bound fractions was calculated from two sets of blots, each done in triplicate. Values are mean and standard deviation.