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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Bone. 2009 Aug 11;45(6):1161–1168. doi: 10.1016/j.bone.2009.08.008

Appendix.

Formulae used to calculate derived bone histomorphometric indices from primary measurements

Abbreviation
Structural		 Parameter Unit Formula
   BV/TV Bone volume/tissue
volume		 % Bone area/tissue area *
100
Static Formation
   OV/BV Osteoid volume/bone
volume		 % Osteoid area/bone area
*100
   OS/BS Osteoid surface/bone
surface		 % Osteoid perimeter/bone
perimeter * 100
   O.Th Osteoid thickness µm (Osteoid area/osteoid
perimeter) * 2/1.2
Dynamic Formation
   MS/BS Mineralizing
surface/bone surface		 % (Double label perimeter +
1/2 single label
perimeter)/bone perimeter
*100
   MAR Mineral apposition
rate		 µm/d (Distance between
labels/inter-label
period)/1.2
   AjAr Adjusted apposition
rate		 µm/d (MAR * MS/BS)/OS/BS *
0.01
   MLT Mineralization lag
time		 d O.Th/AjAr
   OMT Osteoid maturation
time		 d O.Th/MAR
   BFR/BS Bone formation
rate/bone surface
volume		 μm2/mm3/yr MAR * MS/BS * 3.65
Immunohistochemistry
   FGF-23/T.Ar Number of osteocytes
expressing FGF-
23/tissue area		 #/mm2 Number of osteocytes
expressing FGF-23/tissue
area