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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: J Neurosci Res. 2010 Jan;88(1):143–154. doi: 10.1002/jnr.22172

Figure 2.

Figure 2

Effect of memantine in neuroblastoma cells: Analysis of sAPP levels by western blot at different days

Cells were treated with memantine at the indicated concentrations for 12 days, with media samples collected at 3 day intervals and subjected to SDS-PAGE and Western blotting. A-D: Densitometric analysis of Western blot data shows that levels of sAPP in conditioned medium samples collected at day 3 (A) was not significantly changed by memantine. However, sAPP in the day 6 samples (B) and day 9 samples (C), were significantly lower in cultures treated with 2 μM and 4 μM memantine, compared to vehicle (all p<0.05). In the day 12 samples (D), statistically significant decreases in sAPP were observed for all three memantine concentrations (all p<0.05). E: A representative Western blot of day 12 CM samples probed with anti-APP (22C11) and anti-β-actin (AC-15) monoclonal antibodies.