Figure 1. Generation of sRen Knock-out Mouse Model.

Cre-LoxP strategy was used to target exon-1a of mouse Ren-1^c gene using the targeting vector shown. Homologous recombinant founder mice containing the targeted allele were bred with FLpase transgenics to generate the floxed allele. The null allele was obtained by breeding with E2A-Cre transgenic mice. The frt (gray rectangle) and loxP (black triangles) sites are indicated. Exon 1b is shaded black and gray. The final null allele is shown along with the expected sizes from EcoR1 and HindIII digestion and use of the 5′ and 3′ probes (crosshatched boxes). The restriction site indicated in bold are those present in genomic DNA whereas those in italics are unique to the null allele and engineered at the loxP site.