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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Hypertension. 2009 Oct 12;54(6):1240–1247. doi: 10.1161/HYPERTENSIONAHA.109.138677

Figure 2. Southern Blotting of Null and Littermate Controls.

Figure 2

5′ and 3′ Southern probes were employed to confirm correct targeting of Ren-1c exon1a. HindIII digestion produces a 9765 bp wildtype (WT) band and an 8248 bp null band with the 5′ probe. EcoRI digestion generates an 8756 bp WT band and a 6592 bp null band using the 3′ probe. The position of the probes were shown in Figure 1.