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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Arch Biochem Biophys. 2009 Sep 13;491(1-2):69–74. doi: 10.1016/j.abb.2009.09.003

Figure 3.

Figure 3

Empirical evaluation of the reaction order for Co2+ in the uptake reaction with human apo-MnSOD. Human apo-MnSOD apoprotein (25 μg/mL in 20 mM HEPES pH 8.2) was equilibrated to 37°C and metal binding initiated by addition of CoCl2 (50 μM – 1 mM). Metal binding was monitored fluorimetrically as described in the Methods, and the experimental timecourses were analyzed to evaluate the pseudo-first order reaction rate constants. The fitted line has a slope of 1.1±0.01.