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. Author manuscript; available in PMC: 2010 Nov 30.
Published in final edited form as: N Biotechnol. 2009 Jul 1;26(5):244–250. doi: 10.1016/j.nbt.2009.06.982

Figure 4. Identification of an improved inhibitor of NF-κB activation that shows little or no cytotoxicity in Jurkat cells.

Figure 4

(A) NF-κB-dependent hrGFP expression in stably transfected Jurkat cells was stimulated with 20 ng/ml TNFα. In parallel, cells were incubated for 24 h without or with the indicated concentrations of resveratrol or its structural analog, 6q. Cells were harvested and subjected to HyperCyt® flow cytometric analysis. Cells treated with TNFα alone (control) resulted in a mean value of 64. (B) Cells were incubated with resveratrol or 6q as in (A). Following a 24 h incubation, WST-1 reagent was added to cultures, incubated for one additional hour and absorbance measured by spectrophotometry as in figure 3. Cells incubated without luteolin (control) generated a value of 0.37 ± 0.01 absorbance units. Error bars represent standard deviations of triplicate values.