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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Sep 3;1790(12):1665–1672. doi: 10.1016/j.bbagen.2009.08.011

Figure 1.

Figure 1

Figure 1

Figure 1A. MsrA repairs α-crystallin oxidized by HOCl in vitro.

Colloidal blue staining of SDS-PAGE of α-crystallin (5 μg) following CNBr cleavage. Lane 1 – untreated α-crystallin cleaved with CNBr. Lane 2 – oxidized α-crystallin (800 μg oxidized with 1.2 mM HOCl) cleaved with CNBr. Lane 3 - oxidized α-crystallin incubated with MsrA (1.2 mM) and DTT (5 mM) for 1 hr at 37 °C and cleaved with CNBr. Lane 4 - Total α-crystallin standard.

Figure 1B. DTT alone has no effect on HOCl-induced oxidation of α-crystallin.

Colloidal blue staining of SDS-PAGE of α-crystallin (5 μg) following CNBr cleavage. Lane 1 – untreated α-crystallin cleaved with CNBr. Lane 2 – oxidized α-crystallin (800 μg oxidized with 1.2 mM HOCl) cleaved with CNBr. Lane 3 - oxidized α-crystallin incubated with DTT (5 mM) alone for 1 hr at 37 °C and cleaved with CNBr.