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. Author manuscript; available in PMC: 2010 Nov 12.
Published in final edited form as: J Med Chem. 2009 Nov 12;52(21):6897–6911. doi: 10.1021/jm9012054

Figure 2.

Figure 2

Proteome reactivity profiles of three different isothiocyanate (NCS) agents 9–11.

Figure 2A; AB&CR agent-labeled proteins were detected by in-gel fluorescence scanning after Cu(I)-mediated cycloaddition to either a rhodamine-azide reporter probe 39 or a rodamine-alkyne reporter probe 40. Figure 2B; CRMP2 protein was detected by western blot using anti-CRMP2. Figure 2C; All proteins in lysate were visualized by Coomassie blue staining after in-gel fluorescence scanning. (All images shown in gray scale).