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. Author manuscript; available in PMC: 2010 Nov 12.
Published in final edited form as: J Med Chem. 2009 Nov 12;52(21):6897–6911. doi: 10.1021/jm9012054

Figure 3.

Figure 3

In vitro labeling of externally added GST-CRMP2 and endogenous CRMP2 in mixture of mouse lysate and overexpressed GST-CRMP2.

Figure 3A; AB&CR agent-labeled proteins were detected by in-gel fluorescence scanning after Cu(I)-mediated cycloaddition to rhodamine-azide reporter probe 39. Figure 3B; CRMP2 and GST proteins were detected by western blot using anti-CRMP2 and anti-GST antibodies. Figure 3C; All proteins in lysate were visualized by Coomassie blue staining after in-gel fluorescence scanning. (All images shown in gray scale).