[Na]i and pHi in X. laevis oocytes expressing various P-ATPase subunits. Oocytes were injected with various P-ATPase subunit cRNAs, as indicated below the bars. After a 2-day incubation of the oocytes in amphibian Ringer’s solution, [Na]i was measured by using intracellular Na+-selective microelectrodes: results are expressed as mean ± SE; n = 6–25 oocytes, from 2–6 independent experiments. Under the same experimental conditions, pHi was measured by using intracellular pH-selective microelectrodes: results are expressed as mean ± SE; n = 4–7 oocytes, from 2–3 independent experiments. Statistics were performed compared with control (β1 or βg expressing-oocytes) [Na]i value or pHi value. Statistical significance: NS, not significant; ∗∗∗, P < 0.001.