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. Author manuscript; available in PMC: 2010 Dec 3.
Published in final edited form as: J Control Release. 2009 Aug 20;140(2):134–140. doi: 10.1016/j.jconrel.2009.08.008

Figure 4.

Figure 4

Silencing of target d1EGFP expression by various DOTAP/ODN formulations in serum-free medium. CHO-d1EGFP cells received 300nM ODN treatment for a period of 4hr in the absence of serum, and were assayed 24 hours post-treatment. The “no polymer” treatment refers to DOTAP/ODN (charge ratio 4.7) in the absence of polymer, and all other groups refer to the polymer that is added to this combination (to yield a net charge ratio of 1). GFP Fluorescence is normalized to untreated cells (100%). Data represent mean ± s.d. (n=3).