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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Exp Hematol. 2009 Oct 6;37(12):1411–1422. doi: 10.1016/j.exphem.2009.09.009

Figure 2. Proliferation of PV erythroid precursors in response to cytokines under serum-free conditions.

Figure 2

(A) Growth curves of PV erythroblasts plated in serum-free medium in the presence of EPO, SCF or both growth factors in a representative experiment. Cells were counted daily in a hemocytometer using trypan blue exclusion technique and total cell counts were plotted. The data represent mean ± SEM values of triplicate cultures. *P<0.001 (B) Short-term expansion of erythroblasts in serum-free cultures (four days) in response to cooperative effect of EPO and SCF determined by MTT assay. Control group contained no cytokines. Data represent mean ± SEM values (n=6 replicates in each group). *P<0.001. (C) EPO hypersensitivity of PV erythroblasts in serum-free conditions containing decreasing concentrations of EPO ranging as indicated with assessment of proliferation in an MTT assay. Data are represented as % of maximal growth. NS: not significant.