Fig. 6.

(A) Depletion of F4/80⁺ cells in the spleens of wild type mice is caspase-3-dependent. Caspase-1-deficient mice do not show a similar effect. Cells expressing Ly-6G become pyknotic in all three mouse strains. (B) The distribution of Francisella antigens in the livers of wild type and caspase-3-deficient mice infected with KU49. Higher power images of the boxed areas are shown below. Note the similar localization of microbial antigens within granulomas in KU49-infected caspase-3-deficient mice and wild type mice challenged with LVS, which does not induce necrosis of the granulomas (Fig. 1A). Bars, 100 μm.