Table 3.
Relative quantitation of 20 abundant proteins by SILAP standard IEF-2D-LC/MS/MS in CT26 and YAMC secreted proteomes.
| Protein description | Expression ratio CT26/YAMC |
YAMC | CT26 | ||||
|---|---|---|---|---|---|---|---|
| total number of peptides |
xpress ratio mean |
xpress stdev |
total number of peptides |
xpress ratio mean |
xpress stdev |
||
| cathepsin L preproprotein | 88.70 | 86 | 0.1 | 0.06 | 9284 | 8.87 | 3.34 |
| lectin, galactose binding, soluble 1 | 14.37 | 384 | 0.68 | 0.08 | 1614 | 9.77 | 3.29 |
| vimentin | 24.65 | 866 | 0.17 | 0.06 | 1464 | 4.19 | 0.93 |
| enolase 1 | 15.68 | 208 | 0.22 | 0.08 | 1425 | 3.45 | 1.02 |
| cystatin C | 15.73 | 39 | 1.73 | 0.6 | 1392 | 27.22 | 13.66 |
| heat shock protein 8 | 5.13 | 444 | 0.7 | 0.35 | 1360 | 3.59 | 0.98 |
| secreted phosphoprotein 1 | 521.00 | 117 | 0.04 | 0.04 | 1181 | 20.84 | 13.24 |
| pyruvate kinase 3 | 29.11 | 185 | 0.09 | 0.03 | 1022 | 2.62 | 1.19 |
| procollagen, type I, alpha 2 | 334.00 | 31 | 0.04 | 0.02 | 979 | 13.36 | 8.38 |
| profilin 1 | 2.58 | 109 | 1.63 | 0.55 | 971 | 4.21 | 2.72 |
| procollagen C-proteinase enhancer protein | 44.53 | 131 | 0.17 | 0.09 | 915 | 7.57 | 1.97 |
| heterogeneous nuclear ribonucleoprotein A2\B1 isoform 1,2 | 3.02 | 39 | 1.77 | 0.67 | 766 | 5.34 | 2.88 |
| S100 calcium binding protein A4 | 178.67 | 54 | 0.03 | 0.02 | 755 | 5.36 | 2.36 |
| nucleobindin 1 | 21.19 | 159 | 0.21 | 0.09 | 649 | 4.45 | 1.47 |
| eukaryotic translation elongation factor 1 alpha 1 | 4.34 | 193 | 0.5 | 0.12 | 638 | 2.17 | 0.76 |
| heat shock protein 1, beta | 9.21 | 665 | 0.29 | 0.07 | 631 | 2.67 | 0.61 |
| lectin, galactoside-binding, soluble, 3 binding protein | 9.31 | 111 | 0.32 | 0.12 | 454 | 2.98 | 0.6 |
| nucleolin | 14.78 | 122 | 0.27 | 0.14 | 418 | 3.99 | 1.12 |
| fibronectin 1 | 7.23 | 197 | 1.21 | 0.43 | 406 | 8.75 | 3.7 |
| heat shock protein 1, alpha | 4.15 | 601 | 0.62 | 0.27 | 405 | 2.57 | 0.46 |