Table 4.

Relative quantitation of biomarker candidates in Apc^min (Min) and normal mouse serum using SILAP standard LC-MRM/MS. Mean ratio for each protein and ratios for each of two replicates are presented.

Proteins	Normal Sera	Min Sera	Min/Normal Ratio	Normal Sera I			Normal Sera II			Min Sera I			Min Sera II
	Mean Ratio	Mean Ratio		Light	Heavy	Ratio	Light	Heavy	Ratio	Light	Heavy	Ratio	Light	Heavy	Ratio
Cystatin C	0.002	0.025	9.829	187	46933	0.004	60	59679	0.001	981	40571	0.024	628	25256	0.025
Secreted Phosprotein 1	0.002	0.003	1.316	245	71620	0.003	266	207778	0.001	186	87598	0.002	1108	272677	0.004
Profilin 1	0.305	0.155	0.508	1385	4146	0.334	1598	5786	0.276	1351	11588	0.117	939	4854	0.193
Heat shock protein 8	0.147	0.086	0.583	531	3473	0.153	657	4629	0.142	457	6383	0.072	1231	12278	0.100
Pyruvate Kinase 3	0.000	0.059	0.06/0	0	5369	0.000	0	5453	0.000	230	4141	0.056	164	2591	0.063
Procollagen C- proteinase enhancer	0.002	0.003	1.367	0	11561	0.000	83	20573	0.004	64	12296	0.005	14	45111	0.000
Nucleobindin 1	0.005	0.022	4.412	0	2360	0.000	65	6512	0.010	212	8306	0.026	312	16851	0.019
HSP 1, alpha	0.000	0.151	0.15/0	0	2028	0.000	0	1484	0.000	592	4476	0.132	576	3400	0.169
Lectin 3	0.015	0.018	1.232	359	28188	0.013	269	15687	0.017	229	17542	0.013	342	14388	0.024
Nucleolin	0.000	0.008	0.01/0		3051	0.000	0	2888	0.000	116	13736	0.008	94	12026	0.008
Fibronectin 1	1.191	12.227	10.267	1421	921	1.543	828	987	0.839	8415	754	11.160	5836	439	13.294