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. Author manuscript; available in PMC: 2010 Dec 14.
Published in final edited form as: Free Radic Biol Med. 2009 Sep 26;47(12):1742–1752. doi: 10.1016/j.freeradbiomed.2009.09.022

Fig. 2.

Fig. 2

BiP mRNA levels and protein stability do not change in hyperoxia. (A) A549 cells were exposed to room air (RA) or hyperoxia for 24, 36, 48, and 72 hours and total RNA was collected. Reverse transcription followed by quantitative real time PCR was performed using primers specific to BiP. (B) Cells were exposed to RA or hyperoxia for 36 hours and then treated with 50ug/ml cyclohexamide to inhibit translation. Total protein was then collected in 1 hour intervals and BiP protein level was monitored using western blot. (C) Images were scanned and quantified using ImageJ software. BiP protein at all times treated with cyclohexamide was normalized to minus cylcohexamide samples from the corresponding exposure (room air or hyperoxia).