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. Author manuscript; available in PMC: 2010 Dec 14.
Published in final edited form as: Free Radic Biol Med. 2009 Sep 26;47(12):1742–1752. doi: 10.1016/j.freeradbiomed.2009.09.022

Fig. 3.

Fig. 3

Hyperoxia modestly stimulates an ER stress reporter in A549 but not H1299 cells (A) Schematic of the ER stress response element containing portion of the BiP promotor driving transcription of firefly luciferase (BiP(−132-+7)-FLuc). (B) A549 and H1299 cells were transfected with BiP(−132-+7)-FLuc and SV40-RLuc as transfection control. Cells were then placed in room air (RA) or hyperoxia for 48 hours, or DMSO or 0.5ug/ml tunicamycin (TM) for 24 hours. The luciferase activity of cell extracts was measured and data expressed as a ratio of firefly/renilla luciferase activity. The oxygen treated samples were normalized to RA and the TM treated samples normalized to DMSO. Data graphically presented as relative fold change (n=6, *p<0.001).