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. Author manuscript; available in PMC: 2010 Dec 14.
Published in final edited form as: Free Radic Biol Med. 2009 Sep 26;47(12):1742–1752. doi: 10.1016/j.freeradbiomed.2009.09.022

Fig. 8.

Fig. 8

Hyperoxia sensitizes cells to tunicamycin induced cell death. A549 and H1299 cells were exposed to room air plus DMSO vehicle control (RA) or tunicamycin, and hyperoxia plus DMSO vehicle control or tunicamycin for four days. Cell death was assessed using Annexin V / 7AAD staining. (A) Representative histograms are shown for each treatment. (B) Cells that stained positive for either or both Annexin V and 7AAD were counted as dead. Cell death was graphed for each treatment (n=3, *p<0.0001, #p=0.0003). Data are representative of three different experiments with similar results. (C) Whole cell lysates from each treatment were collected and immunoblotted for BiP.