Fig. 9.

Overexpression of BiP does not attenuate cell death from hyperoxia plus tunicaymcin. (A) A549 and H1299 cells were infected with lentivirus encoding for BiP or an empty lentivirus control (termed GFP), creating stably induced cell lines. (A) Infection efficiency was monitored by assessing GFP expression on a per cell basis using flow cytometry. (B) Cells were exposed to hyperoxia or hyperoxia plus 0.5ug/ml tunicamycin. Whole cell lysate was colleceted and immunoblotted for BiP. (C) Cell death was assessed using Annexin V / 7AAD staining. Cells that stained positive for either or both Annexin V or 7AAD were counted as dead. Cell death was graphed for each treatment (n=3, *p<0.0001, #p<0.003). Data are representative of three different experiments with similar results.