Figure 3.
RanBPM alters the agonist-induced subcellular redistribution of MOP. (A) HEK293 cells stably expressing FLAG-MOP ± RanBPM (MOP and MOP/RanBPM) were treated with vehicle (left panels) or 10 μM DAMGO (right panels) for 30 min. FLAG-MOP was visualized with Cy3-conjugated anti-FLAG antibody followed by confocal microscopy. (B) DAMGO-induced internalization of FLAG-MOP was quantified by sucrose density gradient centrifugation assays. See Methods for details. Data are expressed as percent change of receptors in the plasma membrane fraction that shifted to the light vesicle fraction from the untreated heavy fraction following DAMGO treatment. Data are mean ± S.E.M. from at least three experiments. Statistical significance was determined using unpaired Students t-test (*p < 0.05 compared to control).