Skip to main content
. Author manuscript; available in PMC: 2010 Dec 11.
Published in final edited form as: Neurosci Lett. 2009 Sep 27;466(3):154–158. doi: 10.1016/j.neulet.2009.09.048

Table 1.

Receptor density, ligand binding, and inhibition of forskolin-stimulated cAMP accumulation by MOP are unaltered by RanBPM.

Stable Cell Lines Kd (nM) Bmax (fmol/mg) DAMGO EC50 (μM) Maximal Inhibition of cAMP Accumulation (%)
MOP 2.6 ± 0.8 54 ± 5.4 3.4 ± 1.7 78 ± 7.8
MOP+RanBPM 2.8 ± 0.7 49 ± 6.2 2.1 ± 0.8 79 ± 4.3

The binding characteristics (Kd and Bmax) for [3H]naloxone were determined on membranes from HEK293 cells stably transfected with FLAG-MOP ± RanBPM and analyzed by computerized curve fitting (see Materials and Methods). DAMGO-mediated inhibition of cAMP accumulation was assessed as described in the same cells by [3H]adenine labeling and determining percent conversion of [3H]ATP to [3H]cAMP in the presence of 10 μM forskolin and 200 μM IBMX. Data shown are mean ± S.E.M. of three independent experiments each performed in duplicate.