The photolysis efficiency of vesicles and the quantum yield of dyes in the vesicle membrane are anti-correlated. (A) Photolysis efficiencies of vesicles containing different lipids with 2, 3.7, and 5.6 mol% DiD. Vesicles were photolyzed using single 3ns pulses of 550nJ at ~645nm. For each dye concentration and pulse energy, attempted photolysis was performed on 100 vesicles on 3 separate days for a total N=300 for each data point. (B) Quantum yields of vesicles containing different lipids with 2, 3.7, and 5.6 mol% DiD. DMPC:DMPG vesicles in the liquid crystalline phase are held at a temperature of 30 °C. All error bars are reported as standard error.