Figure 7.
The use of a ternary lipid mixture to increase the photolysis efficiency of vesicles. (A) Photolysis efficiencies of vesicles of 9:1 DMPC:DMPG and 9:1 DPPC:DOPC + 10% cholesterol with varying amounts of DiD. All vesicles were photolyzed using single 3ns pulses of 550nJ at ~645nm. For each dye concentration, photolysis was performed on 100 vesicles on 3 separate days for a total N=300 for each data point. (B) Quantum yields of vesicles of 9:1 DMPC:DPPG and 9:1 DPPC:DOPC + 10% cholesterol with varying amounts of DiD. All error bars are reported as standard error. All vesicles were prepared in a 50mM borate buffer at pH 8.5.